Thus, each BSP alone can promote sperm binding and preservation in the oviductal reservoir. Sperm binding to oviductal epithelium is dependent upon the capacitation status of sperm, and only noncapacitated sperm exhibit binding [ 3 , 61 , 62 ]. Sample processing and analysis can have a profound impact on the reliability of the results. Immunoglobulin G in genital secretions is mostly derived from systemic circulation [ 26 ]. The HDL present in the female genital tract facilitate the efflux of cholesterol that occurs during the early steps of capacitation [ 30 , 34 ]. The cells within gate 1 R1 represent the population of spermatozoa.
Anti-Cow (Bovine) Sperm Acrosome Associated Protein 3 antibody for Western Blotting (WB)
anti-Cow (Bovine) Sperm Acrosome Associated Protein 3 antibody for Western Blotting
A total of 5—10 mg of the appropriate anti-BSP antibody in 3 ml PBS was applied to each column, and then the columns were capped and placed on a rotating shaker for 1 h at room temperature to allow antibody binding. However, since both tests are based on counting motile spermatozoa bound to beads or latex particles, the assessment is subjective. Sperm antibodiesreproductive efficiency in the zebu cattle in south-western Nigeria. Acrosome-intact sperm exhibited a homogenous signal over the entire acrosomal region a , and the acrosome-reacted sperm displayed a signal over the equatorial region b.
Spermagglutinins in serum and seminal plasma of bulls and their relationship to fertility classification. Complete amino acid sequence of BSP-A 3 from bovine seminal plasma. It is assumed that syntaxin 2 may be involved in triggering the acrosome reaction through a ligand-receptor mediated signal transduction pathway. Assessment of sperm apoptosis in cryopreserved bull semen after swim-up treatment:
It was speculated that non-mobile antigens were inserted later in germ cell development, and that cross-linking between mobile and non-mobile antigens resulted in loss of capping in late spermatids [ 1 ] and spermatozoa. Gelatin affinity chromatography of BSP proteins produced two peak fractions. In cattle, heparin binds to sperm [ 22 ] and induces changes in the intracellular environment of the sperm. Circulating antisperm antibodies and fertility prognosis: After 10 min at room temperature, samples were washed three times by centrifugation and labeled with fluorescein isothiocyanate FITC -labeled anti-bovine IgG or IgA, or their respective isotype control antibodies, as described below. Discussion Systemic immunization with autologous spermatozoa induced an immune response in all bulls, characterized by an increase in sperm-bound IgG and IgA.